RBRC06047
true
B6-Hes1-floxed ; B6-Hes1 floxed
B6-Hes1-floxed ; B6-Hes1 floxed
Itaru IMAYOSHI
B6.Cg-Hes1<tm1Imayo>
B6.Cg-Hes1<tm1Imayo>
Hes1遺伝子のfloxedマウス。Hes1遺伝子の第一イントロン及び3'UTRの下流に、それぞれLoxPが挿入してある。Cre発現マウスと交配する事で、Hes1のコンディショナルノックアウトを行う事ができる。PGK-Neo配列は除去してある。
今吉 格
TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used. The mutant mice were crossed to C57BL/6 for 6 times.
影山龍一郎
Bacteriophage P1 loxP sites, mouse Hes1 genomic DNA
京都大学ウィルス研究所・今吉 格、影山龍一郎（2007）。TT2 ES細胞由来を用いて作出。B6と交配（6世代）。
<a href='https://brc.riken.jp/mus/pcr06047'>Genotyping protocol -PCR-</a>
C（3〜6か月）
Developmental Biology Research
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Development. 2008 135(15):2531-41.<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>学術機関の学術研究に限る。分与希望者は事前に今吉格(E-mail: iimayosh@virus.kyoto-u.ac.jp)と影山龍一郎 (Emai: rkageyam@virus.kyoto-u.ac.jp)の両方に連絡をとり、分与に関して許可を得ること。（提供承諾書（書式6）の提出は不要）。
C (3-6 months)
Hes1 gene floxed mice. LoxP sequences were knocked into the first intron and downstream of 3'UTR of Hes1 gene for conditional knock-out study. PGK-Neo sequence was removed.
Cre/loxP system
Ryuichiro KAGEYAMA
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2008 135(15):2531-41.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).